ProMAT - Protein Microarray Analysis Tool

The Protein Microarray Analysis Tool (ProMAT) is a data analysis application for protein microarray data. The software was developed for use with ELISA microarray experimental data by the Statistics Group and the Cell Biology and Biochemistry Group at Pacific Northwest National Laboratory. ProMAT takes microarray data (the output of microarray image analysis) where some arrays are treated with standards of known antigen concentration and some arrays are treated with samples of unknown concentration. ProMAT fits standard curves to the standards data to relate spot fluorescence to concentration. ProMAT also calculates and outputs confidence bounds on these standard curves. The tool then uses the standard curves to predict antigen concentrations for the unknown samples, along with prediction intervals.